Antigenic analysis of classical swine fever virus E2 glycoprotein using pig antibodies identifies residues contributing to antigenic variation of the vaccine C-strain and group 2 strains circulating in China

Table 2 Characteristics of three monoclonal antibodies against recombinant E2-AD protein of the vaccine C-strain

			Western blot^a (rE2-AD protein)			IFA^b (virus infected cells)			Antibody binding/neutralization efficiency^c
mAb	Isotype	Epitope	C-strain	QZ-07	HZ1-08	C-strain	QZ-07	HZ1-08	C-strain	QZ-07	HZ1-08
1E7	IgG1	Conformational epitope In antigenic unit B/C	-	-	-	+	+	+	5.3/3.35	3.2/<1.7	2.9/<1.7
2B6	IgG2b	Linear epitope at position 1-110 aa	+	±	±	+	-	-	4.4/<1.7	0/0	0/0
6B8	IgG2b	Conformational epitope in antigenic unit B/C	-	-	-	+	+	+	5.6/4.85	4.4/<1.7	4.1/<1.7

^aValues represent binding of mAbs to denatured prokaryotic-derived rE2 proteins as detected by Western blotting: "+"= strong reactivity; "±"= weak reactivity; "-"= no reactivity detected.
^bValues represent binding of mAbs to native viral E2 proteins detected by immunofluorescence assay: "+"= fluorescent signal detected; "-"= no signal detected.
^cIFA and neutralization assay were performed by serial dilutions of mAbs to assess the antibody binding and neutralization efficiency with different CSFV strains.

ISSN: 1743-422X