Antigenic analysis of classical swine fever virus E2 glycoprotein using pig antibodies identifies residues contributing to antigenic variation of the vaccine C-strain and group 2 strains circulating in China

Table 1 Primers used in PCR amplification of various recombinant E2 proteins

Primer designation^a	Nucleotide sequence^b	Target region of E2 protein^c	CFSV strain amplified	Location in the C-strain genome^d
C-E2-BC-f	5-AAAGGATCC ATGCGCTTAGCCTGCAAGGAAGATTAC	BC unit		2442-2465
C-E2-BC-r	5-AAACTCGAG TCAGAAAGCACTACCG	BC unit		2804-2816
C-E2-AD-f	5-AAGGATCC ATGCGGCTAGCCTGCAAG	BC + AD units	Vaccine C-strain	2442-2456
C-E2-AD-r	5-TAGCTCGAG TCAATCTTCATTTTCCAC	BC + AD units		2955-2969
C-E2-f	5-TTTGGATCC GCCACCATGGTATTAAGGGGACAGATCG	Full-size E2		2379-2397
C-E2-r	5-ATTCTCGAG TCAACCAGCGGCGAGTTGTTCTG	Full-size E2		3541-3560
QZ-E2-AD-f	5-AAAGGATCC CGCCTGTCCTGTAAGG	BC + AD units	Subgroup 2.1 Strains	2442-2457
QZ-E2-AD-r	5-TAGCTCGAG GTCTTCTTTTTCTAC	BC + AD units		2955-2969

^af, forward; r, reverse.
^bUnderline represents the restriction enzyme digestion sites used for cloning.
^cSee Figure 1 for the various regions of E2 protein.
^dLocations are derived from the genome of classical swine fever virus C-strain (GenBank accession no. HM175885).

ISSN: 1743-422X