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Figure 3 | Virology Journal

Figure 3

From: Antigenic analysis of classical swine fever virus E2 glycoprotein using pig antibodies identifies residues contributing to antigenic variation of the vaccine C-strain and group 2 strains circulating in China

Figure 3

Binding efficiency of pig anti-CSFV sera with different rE2-AD proteins. (A) Binding of the rE2-AD proteins from the C-strain and eight subgroup 2.1 strains to pig antisera collected at 78 days post immunization with the C-strain or 25 days post infection with strain QZ-07, respectively. For each of the rE2-AD proteins, the binding efficiency was determined by normalizing to anti-His-tag binding first, and then to C-strain protein or strain QZ-07 rE2-AD protein binding for anti-C-strain or anti-QZ-07 sera, respectively. Thus homologous binding efficiency was set at 100%. Error bars represent standard deviation from three separate experiments. (B) Western blots of rE2-AD proteins using pig anti-C-strain serum, pig anti-QZ-07 serum and mouse monoclonal anti-His-tag antibody.

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Virology Journal

ISSN: 1743-422X