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Figure 1 | Virology Journal

Figure 1

From: Antigenic analysis of classical swine fever virus E2 glycoprotein using pig antibodies identifies residues contributing to antigenic variation of the vaccine C-strain and group 2 strains circulating in China

Figure 1

Generation of prokaryotic-derived recombinant (rE2) proteins. (A) Schematic presentation of expression of truncated rE2 proteins of CSFV. The antigenic domain of glycoprotein E2 is marked in grey and three antigenic regions identified in this study are marked with different colors. The rE2-BC and rE2-AD proteins expressed in this study are indicated by arrows. The N-linked glycosylation sites (lollipop structures), three disulfide bonds (s-s), the signal sequence (S) and the transmembrane region (TM) are also shown. (B) Antigenic reactivity of the rE2 proteins. The rE2-BC and rE2-AD proteins of CSFV C-strain were expressed in E. coil, run through SDS-PAGE and analyzed by Western blot analysis using pig hyperimmune serum against CSFV vaccine C-strain. Molecular weight markers (kDa) are indicated to the left of each panel.

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Virology Journal

ISSN: 1743-422X