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Figure 2 | Virology Journal

Figure 2

From: Differences in the ability to suppress interferon β production between allele A and allele B NS1 proteins from H10 influenza A viruses

Figure 2

IFN-β release in the supernatant and expression of IFN-β m-RNA in human A549 epithelial cells in response to poly I:C challenge at the presence of different NS1 proteins. (A) The concentration of IFN-β in A549 cell supernatants was assayed. Cell were transfected with plasmids containing either the NS gene of "mink/84" or "chicken/49" virus or was mock treated, 24 hours later cells were stimulated with 5 μg/ml of poly I:C. The cell supernatants were collected at 0, 2, 4, 8, 16, 24 and 48 hours post-poly I:C stimulations. Expression of IFN-β m-RNA in A549 cells in response to poly I:C challenge at the presence of different NS1 proteins. A549 cells were transfected with (B) empty pCDNA-3 vector, (C) pNS-mink/84 and (D) pNS-chicken/49 respectively, 24 h later cell were treated with 5 μg/ml poly (I:C) for indicated time. Data are expressed as the mean value for the three independent experiments performed in duplicate.

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Virology Journal

ISSN: 1743-422X