Table 2 Intra- and inter-assay reproducibility of the real-time PCR
From: Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR
Concentration of plasmid Standard (copy/μl) | Intra-assay | Inter-assay | ||||
|---|---|---|---|---|---|---|
| Â | Mean Ct | SD | CV (%) | Mean Ct | SD | CV (%) |
107 | 17.88 | 0.1069 | 0.68 | 17.69 | 0.7498 | 4.2 |
105 | 24.49 | 0.2589 | 1.05 | 24.03 | 0.5597 | 2.3 |
103 | 30.98 | 0.2092 | 0.59 | 30.22 | 0.5632 | 1.9 |