Table 9 After four days differentially expressed genes (DEGs) primer sequences for quantitative real time PCR
Name of genes | Sequences of primers | MERS |
|---|---|---|
Primer sequences four days post inoculation of βC1 of ChLCB for Q-RTPCR analysis | ||
SA1 | SA1 F: GTCACCCGGGATGTGACGCCGACGGTCAAT | (30 MERS) |
SA1 QPCR: GGGCCGCACCATGGTCCTGCTGACTTACCG | (30 MERS) | |
SA1 F2: GACGGTCAATCCATGTAT | (18 MERS) | |
SA1 QPCR2: GGTGTCAGGAGACCCCTTCCA | (17 MERS) | |
SA1 F3: GGTAGAGCCCCAGTCTTCCA | (20 MERS) | |
SA1 QPCR3: GCACCCGCCCAACTCCACGG | (17 MERS) | |
SA2 | SA2 F: AATACCCGGGATGATAAACATTTGGGGG | (30 MERS) |
SA2 QPCR: CCAATGTCTAGTCTTGATGCAAAATCAA | (30 MERS) | |
SA2 F2: CTAGTAAAGTTTTATGGATTCTTGGA | (17 MERS) | |
SA2 QPCR2: ATGGATAATAGGGTGATCAGT | (17 MERS) | |
SA2 F3: CACTTGGACTGTGGTCCTG | (18 MERS) | |
SA2 QPCR3: GTCAGCCACCTTAGCTCG | (20 MERS) | |
SA3 | SA3 F: CGCGCCCGGGATGCATCTAGATTGTCCACA | (30 MERS) |
SA3 QPCR: TCAATCAGACGCGAGGTTAAGGTTTCAGAC | (30 MERS) | |
SA3 F2: GAAGGCTATGTAAACGAG | (18 MERS) | |
SA3 QPCR2: GCTCTTCAAGGGTCGGGTTCAG | (17 MERS) | |
SA3 F3: GACTTGGTCGTCGCTGGTA | (20 MERS) | |
SA3 QPCR3: GCTTGATCGCGTACAGG | (18 MERS) | |
SA4 | SA4 F: GCGACCCGGGAtGCATCTAGATTTGGGGGA | (30 MERS) |
SA4 QPCR: AGAAACAGAAGATCTCTGGCTCAGTTTAGG | (30 MERS) | |
SA4 F2: TTCATGATTGTTGGCGCAC | (18 MERS) | |
SA4 QPCR2: CTGATCTTCCTGTGGA | (17 MERS) | |
SA4 F3: CGGCATGACCCTGTGTAA | (20 MERS) | |
SA4 QPCR: GGGGGACTCGCGCCAGG | (17 MERS) | |