Table 4 Relative quantification methods of differentially expressed genes four days post inoculation.
Gene | Identity | Relative quantification against Unit mass | Relative quantification normalized to a reference gene | |||
|---|---|---|---|---|---|---|
Control | Healthy | Livak method | ΔCT Method | Pfaffi Method | ||
SA1 | Trigger factor (chaperone in protein export) | 2.88 | 17.75 | 1.443C/7.727H | 1.08C/1.74H | 1.443C/7.727H |
SA2 | A. thaliana calmodulin-binding receptor-like kinase | 3.759 | 3.759 | 8.564 | - | 8.564 |
SA3 | Polyribonucleotide nucleotidyltransferase | 1.32 | 1.70 | 0.664C/0.742H | 0.790C/.837H | 0.664C/0.742H |
SA4 | Chromosomal replication initiator protein DnaA | 0.659 | 0.882 | 0.329C/0.253H | 0.674C/0.706H | 0.329C/0.253H |