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Figure 3 | Virology Journal

Figure 3

From: Soluble CD40 ligand-activated human peripheral B cells as surrogated antigen presenting cells: A preliminary approach for anti-HBV immunotherapy

Figure 3

HBV core peptide was loaded on sCD40L-activated B cells. As negative control, auto fluorescence of CD40-B cells and isotype control are shown. (a) To gate CD19 positive cells by FACS analysis, the green curve was isotype control and the red curve was B cells specific binding of HBV core peptide. The y-axis shows relative cell number and the x-axis shows the fluorescence intensity of the cells. (b) To observe the B cells specific binding of HBV core peptide by fluorescence microscope, the red fluorescence located at B cell surface was CD19-PE and the yellow fluorescence (superimposition of the green FITC fluorescence and the red CD19-PE becomes yellow) located in cytoplasm was HBV core protein (FITC-FLPSDFFPSV).

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Virology Journal

ISSN: 1743-422X