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Table 3 Oligonucleotide sequences of primers and probe used in AHV-1 FQ-PCR detection

From: Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe

Name

Type

Sequences (5' to 3')

Length

(nt)

Amplicon size

(bp)

P1

Forward

CGGAATTC CAAAACGCCGCACAGATGAC

28

1296

P2

Reverse

CCCTCGA GGTATTCAAATAATATTGTCTGC

30

 

P3

Forward

GAAGGACGGAATGGTGGAAG

20

78

P4

Reverse

AGCGGGTAACGAGATCTAATATTGA

25

 

P

Probe

FAM-CCAATGCATCGATCATCCCGGAA-TAMRA

23

 
  1. The underlined sequences of P1 and P2 are Eco R I and Xho I restriction sites respectively