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Table 1 Intra-assay and inter-assay of the TaqMan™ FQ-PCR assay

From: Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe

Variation Copies of standard Crossing point
   Mean SDa CV (%)b
Intra-assay 1.00E+08 19.73 0.15 0.77
  1.00E+07 23.10 0.20 0.87
  1.00E+06 26.37 0.29 1.09
  1.00E+05 29.63 0.21 0.70
  1.00E+04 33.07 0.31 0.92
  1.00E+03 36.33 0.31 0.84
  1.00E+02 39.50 0.17 0.44
  1.00E+01 42.67 0.32 0.75
Inter-assay 1.00E+08 19.70 0.28 1.41
  1.00E+07 23.09 0.47 2.03
  1.00E+06 26.31 0.32 1.23
  1.00E+05 29.59 0.42 1.42
  1.00E+04 33.04 0.34 1.03
  1.00E+03 36.35 0.41 1.14
  1.00E+02 39.52 0.43 1.10
  1.00E+01 42.70 0.41 0.97
  1. Repeatability and reproducibility (R & R) of the TaqMan™ FQ-PCR assay.
  2. a Standard deviation.
  3. b Coefficient of variation.