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Table 1 Intra-assay and inter-assay of the TaqMan™ FQ-PCR assay

From: Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe

Variation

Copies of standard

Crossing point

  

Mean

SDa

CV (%)b

Intra-assay

1.00E+08

19.73

0.15

0.77

 

1.00E+07

23.10

0.20

0.87

 

1.00E+06

26.37

0.29

1.09

 

1.00E+05

29.63

0.21

0.70

 

1.00E+04

33.07

0.31

0.92

 

1.00E+03

36.33

0.31

0.84

 

1.00E+02

39.50

0.17

0.44

 

1.00E+01

42.67

0.32

0.75

Inter-assay

1.00E+08

19.70

0.28

1.41

 

1.00E+07

23.09

0.47

2.03

 

1.00E+06

26.31

0.32

1.23

 

1.00E+05

29.59

0.42

1.42

 

1.00E+04

33.04

0.34

1.03

 

1.00E+03

36.35

0.41

1.14

 

1.00E+02

39.52

0.43

1.10

 

1.00E+01

42.70

0.41

0.97

  1. Repeatability and reproducibility (R & R) of the TaqMan™ FQ-PCR assay.
  2. a Standard deviation.
  3. b Coefficient of variation.