The amplification curves (Figure 1.a.) and standard curve (Figure 1.b.) of the TaqMan™ FQ-PCR detection. Ten-fold dilutions of standard DNA ranging from 1.0 × 108 to 1.0 × 101 copies/reaction were used (1-8), as indicated in the x-axis, whereas the corresponding Ct values are presented on the y-axis. The correlation coefficient and the slope value of the regression curve were calculated and indicated.