Figure 6

BTV-1 infection of HeLa cells activates NF-κB response. A: HeLa cells were transfected either pGL3Basic luciferase control or pGL3NFκB luciferase and infected with BTV-1 (MOI 0.5-1) or treated with DOX and analysed for luciferase activity (RLU). Normalised RLU values for the control uninfected (grey), DOX treated (white), BTV-1 (black) and BTV-1 in the presence of SN50 inhibitor (black-lined) at different time points (0 h-16 h) are shown. B: Expression of luciferase (RLU) from an NF-κB dependent luciferase reporter in the presence of individual BTV proteins after induction with poly I:C assay at two different times, 6 h (a) and 24 h (b). The normalised RLU was measured in uninduced (white) and induced (black) and the fold induction (<0 = suppression, 0 = no change, >0 = induction) was calculated as indicated. This is a representative experiment and the data presented is normalised from 3 different tests.