Figure 4

Inhibition of NF-κB with SN50 inhibitor does not prevent apoptosis in BTV infected cells. A: Morphological changes associated with BTV infection in the presence or absence of SN50 inhibitor at different times p.i. BTV infected cells and uninfected cells without the inhibitor and BTV infected cells and uninfected cells in the presence of the SN50 inhibitor are also shown. B: NF-κB induction by BTV-1 infection of mammalian cells does not influence the cleavage of caspase-3. Immunoblot analysis of BTV-1 infected, BTV-1 infected that had been pre-treated with 32 uM of SN-50 inhibitor, uninfected and poly I:C treated HeLa cells was undertaken on whole cell lysate at different times p.i and the cleavage of caspase-3 detected with rabbit anti-caspase-3. The cleaved 17 kDa product is shown.