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Figure 3 | Virology Journal

Figure 3

From: Role of cellular caspases, nuclear factor-kappa B and interferon regulatory factors in Bluetongue virus infection and cell fate

Figure 3

BTV causes an apoptotic response in preference to a necrotic response in mammalian cell culture. A: The detection of a second executioner, caspase-7, in BTV-1 infected cells. Whole cell lysate extracts were prepared from different times post infection and monitored for caspase-7 cleavage with a specific anti-caspase-7 polyclonal antibody. B: Detection of caspase-dependent PARP cleavage in BTV infection by immunoblot at different times p.i. Cleavage of the PARP from the full length (116 KDa) to inactive cleaved form (85 kDa) is observed in the BTV infected and DOX treated cells and not the control uninfected. C: Detection of HMGB-1 (26 kDa) by immunoblot in the nuclear (left) and cytosolic (right) fractions of HeLa cells that were uninfected (1), DOX-treated (2) and BTV-1 infected (3).

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Virology Journal

ISSN: 1743-422X