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Figure 2 | Virology Journal

Figure 2

From: Impaired antiviral activity of interferon alpha against hepatitis C virus 2a in Huh-7 cells with a defective Jak-Stat pathway

Figure 2

Replication of JFH1-GFP full-length RNA and JFH1-GND-GFP mutant RNA in Huh-7.5 cells after transfection. Huh-7.5 cells were electroporated with 10 μg of in vitro transcribed RNA prepared either from full-length or GND mutant plasmid. Intracellular expression of GFP in the transfected culture was examined under a fluorescence microscope. (A) Intracellular GFP expression in Huh-7.5 cells transfected with JFH1-GFP RNA at 0, 24, 48, 72 and 96 hours. (B) Intracellular expression of GFP in Huh-7.5 cells transfected with JFH1-GND-GFP mutant RNA at 0, 24, 48, 72 and 96 hours. (C) Intracellular GFP expression measured by flow cytometry in the Huh-7.5 cells transfected with JFH1-GFP RNA after 72 hours. (D): Intracellular GFP expression measured by flow cytometry in the transfected cells of JFH1-GND-GFP mutant RNA after 72 hours.

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Virology Journal

ISSN: 1743-422X