Table 1 DNA Replication Proteins Encoded by Bacteriophage T4

Replicase

   gp43 DNA polymerase

DNA directed 5' to 3' DNA polymerase

   gp45 protein

Polymerase clamp enhances processivity of gp43 polymerase and RNase H

   gp44/62 protein

clamp loader utilizes ATP to open and load the gp45 clamp

   gp32 protein

cooperative single stranded DNA binding protein assists in unwinding duplex

Primosome

   gp41 helicase

processive 5' to 3' replicative helicase

   gp61 primase

DNA dependent RNA polymerase generates lagging strand RNA pentamer primers in concert with gp41 helicase

   gp59 protein

helicase assembly protein required for loading the gp41 helicase in the presence of gp32 protein

Lagging strand repair

   RNase H

5' to 3' exonuclease cleaves Okazaki RNA primers

   gp30 DNA ligase

ATP-dependent ligation of nicks after lagging strand gap repair