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Figure 1 | Virology Journal

Figure 1

From: Quantitative PCR used to Assess HIV-1 Integration and 2-LTR Circle Formation in Human Macrophages, Peripheral Blood Lymphocytes and a CD4+ Cell Line

Figure 1

Quantitation of HIV-1 integration and 2-LTR circle formation in CD4+ U373 cells. U373-MAGI-CCR5 cells were plated in 6-well plates with or without raltegravir treatment 24 h prior to infection and during infection (MOI = 0.1). Two days after infection, (A) β-galactosidase activity (expressed as RLU = Relative Light Units) was analyzed for determination of HIV-1SX infection; (B) cellular genomic DNA was extracted from U373 cells 48 h after infection and HIV-1SX integration was detected using two-step quantitative PCR, and (C) 2-LTR circle formation was measured by real-time PCR. (**p < 0.01)

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