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Table 1 Coefficients of variation for the real-time PCR for PPV-NS1 performed for four concentrations of the standard plasmid at one time (one block with four replications per concentration) or at four different times (four blocks).

From: Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene

Within one block or among four blocks Concentration of standard plasmid (copies/μL) n Ct
(mean)
S.D. CV (%)
Within 6.0 × 108 4 17.67 0.38 2.15
Within 6.0 × 106 4 24.38 0.53 2.17
Within 6.0 × 104 4 30.23 0.44 1.46
Within 6.0 × 102 4 37.18 0.36 0.97
Among 6.0 × 108 4 17.35 0.04 0. 21
Among 6.0 × 106 4 24.30 0.14 0. 57
Among 6.0 × 104 4 30.83 0.14 0. 46
Among 6.0 × 102 4 37.80 0.16 0. 42