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Table 1 Coefficients of variation for the real-time PCR for PPV-NS1 performed for four concentrations of the standard plasmid at one time (one block with four replications per concentration) or at four different times (four blocks).

From: Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene

Within one block or among four blocks

Concentration of standard plasmid (copies/μL)

n

Ct

(mean)

S.D.

CV (%)

Within

6.0 × 108

4

17.67

0.38

2.15

Within

6.0 × 106

4

24.38

0.53

2.17

Within

6.0 × 104

4

30.23

0.44

1.46

Within

6.0 × 102

4

37.18

0.36

0.97

Among

6.0 × 108

4

17.35

0.04

0. 21

Among

6.0 × 106

4

24.30

0.14

0. 57

Among

6.0 × 104

4

30.83

0.14

0. 46

Among

6.0 × 102

4

37.80

0.16

0. 42