Figure 4
From: Characterization of viroplasm formation during the early stages of rotavirus infection
The amount of NSP2 protein increases with time of infection. MA104 cells were infected with RRV at the indicated MOI, and at different times post-infection at 37°C, the cells were harvested in Laemmli buffer. Equal amount of cell lysates were separated by SDS-PAGE and blotted onto nitrocellulose. (A) The expression of rotavirus NSP2 protein was determined by immunostaining with a rabbit antibody to NSP2 and a goat anti-rabbit antibody coupled to peroxydase. A representative experiment from three carried out is shown. (B) Optical density of the protein bands shown in A, as determined by scanning and analysis using Image pro.