Figure 3

The proportion of larger viroplasms increases during rotavirus infection. (A) MA104 cells were infected at different MOI's as described in Figure 1, and the area of each viroplasm was estimated by pixel determination using Image J. The same images used in Figure 2 were analyzed for this figure. Each value represents the mean ± standard error of viroplasms detected in 360 cells, in pixels^2. (B) Based on a microscopic comparison, viroplasms were divided into three arbitrary groups, small (S) (4-33 pixels²), medium (M) (34-69 pixels²), and large (L) (70-200 pixels²). Arrows point to viroplasms representative of each size S, M, and L. (C) Relative amounts of small, medium and large viroplasms during the course of infection at different MOI's. Bars represent the proportion of viroplasms for each multiplicity of infection, (black bars - small; white bars medium; grey bars large viroplasms) with 100% being the total number of viroplasms counted in 360 cells. The numbers under each graph represent the number of the different classes of viroplasms found in each condition analyzed.