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Figure 5 | Virology Journal

Figure 5

From: Targeting lentiviral vector to specific cell types through surface displayed single chain antibody and fusogenic molecule

Figure 5

Study of the entry mechanism of engineered lentiviral vector for transducing target cells. (A) 293T/CD20 or Ghost-CCR5 cells were pre-incubated with various amount of bafilomycin for 30 minutes and spin-transduced with FUGW/SC2H7-A2/SGN or FUGW/GP160. Cells were incubated for an additional 3 hours before replenishing with fresh media. Transduction efficiency was measured by FACS analysis of GFP-positive cells 3 days post-transduction. All data was normalized to transduction without bafilomycin treatment. (B) Effects of supplement of soluble αCD20 on targeted transduction. 293T/CD20 cells were incubated with FUGW/SC2H7-A2/SGN or FUGW/VSVG and various amounts of soluble αCD20 or isotype control for 12 hours, after which the medium was replaced with fresh medium. Transduction efficiency was measured by FACS analysis of GFP-positive cells 3 days post-transduction. All data was normalized to transduction without soluble antibody treatment.

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