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Figure 3 | Virology Journal

Figure 3

From: Targeting lentiviral vector to specific cell types through surface displayed single chain antibody and fusogenic molecule

Figure 3

Incorporation of both FM and antibody onto the vector surface. (A) 293T (top) and 293T/CD20 (bottom) cells were incubated with either FUGW/SC2H7-A2/SGN (left) or FUGW/VSVG (right) at 4°C for 1 hour, fixed and immunostained with anti-p24 antibody (green) and DAPI nuclear staining (blue). Images were acquired with a laser scanning confocal microscope. Scale bar represents 2 μm. (B) Co-expression of antibody and FM on the same viral surface. 293T (shaded area) or 293T/CD20 (solid line) cells were incubated with FUGW/SC2H7-A2/FM, FUGW/SC2H7-GS/FM or FUGW/AB/FM at 4°C for 1 hour, followed by staining of FM by anti-HA antibody. The binding of the virus to the cells was detected by FACS analysis.

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Virology Journal

ISSN: 1743-422X