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Figure 2 | Virology Journal

Figure 2

From: Targeting lentiviral vector to specific cell types through surface displayed single chain antibody and fusogenic molecule

Figure 2

Co-transfection of virus-producing cells to generate targeting lentiviral vectors. 293T cells were transiently transfected with, FUGW, pSC2H7-A2, pFM and the other standard packaging plasmids (pMDLg/pRRE and pRSV-Rev) to make FUGW/SC2H7-A2/FM. Antibody construct pSC2H7-GS was used to generate FUGW/SC2H7-GS/FM. An isotype control antibody construct pAB was used in the transfection to produce non-targeting lentiviral vector FUGW/AB/FM. Transfection with plasmids encoding VSVG was used to generate a control vector, FUGW/VSVG. (A) FACS analysis of GFP expression on transfected cells. Solid line, analysis on transfected 293T/CD20 cells; shaded area, analysis on 293T cells. (B) Analysis of co-expression of the FM and antibody on gated GFP-positive cells. FM was stained using an anti-HA antibody and antibody was stained using an anti-human IgG antibody.

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Virology Journal

ISSN: 1743-422X