Table 1 Sample spreadsheet of data analysis using the 2-ΔΔCt method
From: Identification and characterization of duck plague virus glycoprotein C gene and gene product
Time | gC | β-actin | ΔΔCt | 2-ΔΔCt | Log | ||
|---|---|---|---|---|---|---|---|
|  | MeanCt,TimeX | MeanCt,Time0 | MeanCt,TimeX | MeanCt,Time0 |  |  | (2-ΔΔCt) |
1 h | 21.3 | 24.5 | 10.6 | 13.7 | -0.1 | 1.071773 | 0.03 |
4 h | 20.7 | 24.5 | 11.3 | 13.7 | -1.4 | 2.639015 | 0.42 |
7 h | 19.6 | 24.5 | 12.5 | 13.7 | -3.7 | 12.99603 | 1.11 |
10 h | 16.5 | 24.5 | 12.5 | 13.7 | -6.8 | 111.4304 | 2.05 |
14 h | 14.1 | 24.5 | 10.7 | 13.7 | -7.4 | 168.897 | 2.23 |
20 h | 14.6 | 24.5 | 12.4 | 13.7 | -8.6 | 388.0234 | 2.59 |
28 h | 11.3 | 24.5 | 17.9 | 13.7 | -17.4 | 172950.5 | 5.24 |
36 h | 11.9 | 24.5 | 18.5 | 13.7 | -17.4 | 172950.5 | 5.24 |
54 h | 14.4 | 24.5 | 19.8 | 13.7 | -16.2 | 75281.1 | 4.88 |
72 h | 18.9 | 24.5 | 24 | 13.7 | -15.9 | 61147.25 | 4.79 |