Figure 4
From: Identification and characterization of duck plague virus glycoprotein C gene and gene product
Expression, purification and antigenicity analysis of pET32a-gC recombinant fusion protein. (a) Expression of pET32a-gC recombinant fusion protein. Lanes: 1, pET32a-gC, non-induced; 2, pET32a-gC, induced by 0.6 mmol/L IPTG; 3, pET32a-gC, induced by 1 mmol/L IPTG; 4, IPTG-induced inclusion body fraction. (b) Purification and antigenicity analysis of the recombinant fusion protein. Lanes: 1, Purification of the fusion protein by electric elution; 2, Western blot result of pET32a-gC recombinant fusion protein. M, Protein molecular mass markers.