Figure 1

Interaction between Meq and p53. (A) Schematic representation of the wild-type Meq protein (Meq) and the Meq protein of the deletion mutant (Meq-Δp53BD), which lacked the p53 binding region. The numbers indicate amino acid positions. (B) Yeast AH109 cells were transformed with a combination of the indicated plasmids and selected on low-stringency and high-stringency media. (C) CEF cells were transfected with a combination of the indicated plasmids and incubated for 24 h. Flag-tagged influenza virus M1 protein (Flag-M1) was used as a negative control. The cell lysates prepared from the transfectants were subjected to immunoprecipitation using anti-Flag antibodies. The immunoprecipitates were immunoblotted with anti-GFP antibodies. The cell lysates were included as a loading control. IP, immunoprecipitation. WB, Western blot. (D) CEF cells were co-transfected with Flag-Meq and GFP-p53 and incubated for 24 h. The transfectants were fixed in a 1:1 solution of methanol/acetone for 20 min at -20°C and immunostained with anti-Flag antibodies (panel a, red). The cells were also stained for DNA with 4',6'-diamidino-2-phenylindole (DAPI) (panel d, blue). Panel c shows the merged images of panels a and b (green). Bar, 5 μm.