Figure 5
From: Tracking hantavirus nucleocapsid protein using intracellular antibodies
Titration of viral load in cell culture supernatants by TCID50 assay. Intracellular antibody-expressing stable cell lines were infected with hantavirus 76-118 and L99 respectively; virus titer at sixth day post infection was determined by the TCID50 assay. Endpoint was determined on day 10, cells fixed on the bottom of the culture plate by 80% (v/v) ice-cold acetone. Viral antigen were detected by horseradish peroxidase conjugated L13F3 monoclonal antibody, virus infection was assessed by absorbance at 450 nm (reference 620 nm). Error bars represent the standard deviation of three independent experiments.