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Table 8 Primers used in amplification of the non-coding region of different ISAV segments using cDNA from ligated vRNA as template

From: Identification of the 3' and 5' terminal sequences of the 8 rna genome segments of european and north american genotypes of infectious salmon anemia virus (an orthomyxovirus) and evidence for quasispecies based on the non-coding sequences of transcripts

ISAV RNA segment

Primer sequence

Segment 2

Forward: 5'-GTCAGCCACGCCTACAGTT-3'

 

Reverse: 5'-GCTGCAGCATAGAGTTCGAT-3'

Segment 3

Forward: 5'-CTTCATCATCTACACCAGCCA-3'

 

Reverse: 5'-GCAAGTGAACGTGACTGACA-3'

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Virology Journal

ISSN: 1743-422X