Skip to main content

Table 7 Reverse primers1 used in 5'RACE for amplification of 5' non-coding region of different ISAV segments using vRNA as template

From: Identification of the 3' and 5' terminal sequences of the 8 rna genome segments of european and north american genotypes of infectious salmon anemia virus (an orthomyxovirus) and evidence for quasispecies based on the non-coding sequences of transcripts

ISAV RNA segment

Primer sequence

Segment 1

ssp1: 5'-GTAGTGGCAAGTGCTAGATAC-3'

 

ssp2: 5'-GCCATGAGGTGCTGCATTG-3'

Segment 2

ssp1: 5'-GAAGCTGTGAACAGAGGACAT-3'

 

ssp2: 5'-GCTGCAGCATAGAGTTCGAT-3'

Segment 3

ssp1: 5'-GGTACAGATCCAACATCAAG-3'

 

ssp2: 5'-GCAAGTGAACGTGACTGACA-3'

Segment 4

ssp1: 5'-CTCTGTGCAAACAAAAGCAGA-3'

 

ssp2: 5'-TGGCAACATGACCCTGAGCT-3'

Segment 5

ssp1: 5'-GAGGAGGATTGCCTATATGAAC-3'

 

ssp2: 5'-CAAGTGGAAATCGGACTAATAG-3'

Segment 6

ssp1: 5'-GATGAGTACGTTGACACAC-3'

 

ssp2: 5'-CAGAGGCACTGACATGTCCA-3'

Segment 7

ssp1: 5'-CACTTGCCCAGACGAGTAC-3'

 

ssp2: 5'-CAGTGACAAGGACGAGATGC-3'

Segment 8

ssp1: 5'-GATCAGTCGAGCGACGATGA-3'

 

ssp2: 5'-GCATCCTGCTCGACAGAGAA-3'

  1. 1The forward primer for PCR in all cases (i.e., with either ssp1 or ssp2) was the Universal Amplification Primer (UAP) that comes with the 5'RACE kit (Invitrogen).
Back to article page

Virology Journal

ISSN: 1743-422X