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Table 6 Reverse primers1 used in 3'RACE for amplification of 3' non-coding region of different ISAV segments using vRNA as template

From: Identification of the 3' and 5' terminal sequences of the 8 rna genome segments of european and north american genotypes of infectious salmon anemia virus (an orthomyxovirus) and evidence for quasispecies based on the non-coding sequences of transcripts

ISAV RNA segment

Primer sequence

Segment 1

ssp4: 5'-GCTGGTTCTGTTGAAGGACAT-3'

Segment 2

ssp4: 5'-GTCAGCCACGCCTACAGTT-3'

Segment 3

ssp4: 5'-CTTCATCATCTACACCAGCCA-3'

Segment 4

ssp4: 5'-CATGTCTAGCACTCCGTGTG-3'

Segment 5

ssp4: 5'-CACTGCACAGTTCTCCATCT-3'

Segment 6

ssp4: 5'-GTCGGTATGATCATGTCGTTA-3'

Segment 7

ssp4: 5'-CATACGACCAGATCATCACTG-3'

Segment 8

ssp4: 5'-CATCGTCGCTCGGCTGATC-3'

  1. 1The forward primer for PCR was the Universal Amplification Primer (UAP) that comes with the 3'RACE kit (Invitrogen).