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Table 5 Heterogeneity of ISAV 5' and 3' non-coding regions in viral cRNA1

From: Identification of the 3' and 5' terminal sequences of the 8 rna genome segments of european and north american genotypes of infectious salmon anemia virus (an orthomyxovirus) and evidence for quasispecies based on the non-coding sequences of transcripts

Non-coding region of ISAV RNA segment

Number of clones

Number of different sequences

Heterogeneity Index (HI)

Frequency of sequences

5' end Segment 1

5

2

0.2

1 (1), 1(4)

3' end Segment 1

 

2

0.2

1 (1), 1(4)

5' end Segment 2

5

2

0.6

1 (2), 1(3)

3' end Segment 2

 

4

0.6

1 (1), 1(1), 1(1), 1(2)

5' end Segment 3

5

3

0.4

1 (1), 1(1), 1(3)

3' end Segment 3

 

5

0.8

1 (1), 1(1), 1(1), 1(1), 1(1)

5' end Segment 4

5

2

0.6

1 (2), 1(3)

3' end Segment 4

 

2

0.2

1 (1), 1(4)

5' end Segment 5

5

1

0.0

1 (5)

3' end Segment 5

 

1

0.0

1 (5)

5' end Segment 6

5

2

0.6

1 (2), 1(3)

3' end Segment 6

 

3

0.4

1 (1), 1(1), 1(3)

5' end Segment 7

5

2

0.6

1 (2), 1(3)

3' end Segment 7

 

3

0.4

1 (1), 1(1), 1(3)

5' end Segment 8

5

3

0.4

1 (1), 1(1), 1(3)

3' end Segment 8

 

4

0.6

1 (1), 1(1), 1(1), 1(2)

  1. 1Sequence obtained using the RNA ligation method.
  2. Heterogeneity index (HI) is calculated as: 1-(proportion of clones bearing majority of the sequence). The number preceding the parentheses indicates a sequence while the number within the parentheses indicates the numbers of clones in which that sequence appear.