Skip to main content

Table 3 Primers used in amplification of the non-coding region of different ISAV segments using cDNA from ligated mRNA transcripts as template

From: Identification of the 3' and 5' terminal sequences of the 8 rna genome segments of european and north american genotypes of infectious salmon anemia virus (an orthomyxovirus) and evidence for quasispecies based on the non-coding sequences of transcripts

ISAV RNA segment

Primer sequence

Segment 1

Forward: 5'-CTGATGGATGAATATGGTGT-3'

 

Reverse: 5'-GCTCCAACTGCTGCTCTAC-3'

Segment 2

Forward: 5'-CTTATCGACGAGGTGGAGGT-3'

 

Reverse: 5'-CTCTGAAACCTGTTGCTACTAT-3'

Segment 3

Forward: 5'-GAGTTCGATGAGGACGATGA-3'

 

Reverse: 5'-CTTCTGACAGGATTGCGCTG-3'

Segment 4

Forward: 5'-CAGAAAATTGCAGTCAGTACC-3'

 

Reverse: 5'-CATGTGCTGATCGGTGTTGG-3'

Segment 5

Forward: 5'-GCTGATTGTAGTTGTGTTGGT-3'

 

Reverse: 5'-GTCTGGTACAGAATGGAACG-3'

Segment 6

Forward: 5'-CATGTACAAGTCTAGAGGTAG-3'

 

Reverse: 5'-GCGTTGTCCAGTGTCATCGA-3'

Segment 7

Forward: 5'-GCTTAGGGCTGGACTTCACT-3'

 

Reverse: 5'-GCATGTGTGACAGACCATC-3'

Segment 8

Forward: 5'-GCATCCTGCTCGACAGAGAA-3'

 

Reverse: 5'-CTTTCATCACACAGTAGAGAG-3'