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Table 1 Reverse primers1 used in 3 'RACE for amplification of 3' non-coding region of different ISAV segments using mRNA transcripts as template

From: Identification of the 3' and 5' terminal sequences of the 8 rna genome segments of european and north american genotypes of infectious salmon anemia virus (an orthomyxovirus) and evidence for quasispecies based on the non-coding sequences of transcripts

ISAV RNA segment

Primer sequence

Segment 1

ssp3: 5'-CTGCTATGGAGAGGTGTATG-3'

Segment 2

ssp3: 5'-GACTTGGACCCTCAACAGATC-3'

Segment 3

ssp3: 5'-GCTAGAACATGTGTAGCTGTG-3'

Segment 4

ssp3: 5'-TGGCAACATGACCCTGAGCT-3'

Segment 5

ssp3: 5'-GAGTTCATCAAATGCTGTGGA-3'

Segment 6

ssp3: 5'-GTTCCTCACTGATGCATTGAC-3'

Segment 7

ssp3: 5'-GATGGTCTGTCACACATGC-3'

Segment 8

ssp3: 5'-CTCTCTACTGTGTGATGAAAGA-3'

  1. 1 The forward primer for PCR was the Universal Amplification Primer (UAP) that comes with the 3'RACE kit (Invitrogen).
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Virology Journal

ISSN: 1743-422X