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Figure 3 | Virology Journal

Figure 3

From: Identification of the 3' and 5' terminal sequences of the 8 rna genome segments of european and north american genotypes of infectious salmon anemia virus (an orthomyxovirus) and evidence for quasispecies based on the non-coding sequences of transcripts

Figure 3

A diagram depicting RNA ligation strategy used for ligating ISAV RNA. Total RNA was isolated from ISAV-infected cell lysates and was ligated using RNA Ligase. A cDNA was synthesized from ligated RNA by Reverse Transcriptase using the random primers. PCR was performed on the cDNA template using segment specific primers. The amplified product was gel-purified, cloned in a plasmid and plasmid DNA sequencing was performed.

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Virology Journal

ISSN: 1743-422X