Detection of viral genome in infected tomato leaf samples and the ORFs encoded by Tomato leaf curl virus. (A) Rolling Circle Amplification (RCA) of total DNA for ToLCV-K3/K5 samples using Ø29 polymerase digested by Bam H I (lanes 1, 2), Xba I (lanes 3, 4) and Xho I (lanes 5, 6). (B) RCA of total DNA for ToLCV-CTM digested by Bam H I, EcoR I, Sma I, Nco I, Xmn I and Nde I (lanes 1-6 respectively). M denotes size marker lane. (C) Genome map of Tomato leaf curl virus DNA A showing the sense (AV1and AV2) and complementary strand ORFs (AC1, AC2, AC3, AC4).