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Figure 3 | Virology Journal

Figure 3

From: Human TOP1 residues implicated in species specificity of HIV-1 infection are required for interaction with BTBD2, and RNAi of BTBD2 in old world monkey and human cells increases permissiveness to HIV-1 infection

Figure 3

Permissiveness to HIV-1 infectivity increases in COS-1 cells transfected with BTBD2 silencer construct. A. COS-1 cells transfected with silencer plasmids or controls were subsequently infected with VSV-G/HIV-1/Luc (p24 titer: 440 ng/ml): Uninfected COS-1 cells (Cos1), pSIREN control (S-ve), pSIREN encoding Luciferase shRNA (S-luc), pSIREN encoding BTBD2 shRNA (D2 #7). Bold numbers above bars represent actual average infectivity. B. HIV-1 infectivity in COS-1 cells transfected with either non-specific siRNA or TRIM5α specific siRNA. Bold numbers above bars represent actual average infectivity data. Forty-eight hours post-transfection, transiently transfected and untransfected (COS-1) cells were seeded onto 48-well plates at 20,000 cells/well) and inoculated 24 hours later with 5, 10 and 20 μl of VSV-G/HIV-1/Luc virus in triplicate. Cells were lysed 48 hours after virus inoculation and luciferase activity in the lysates was measured. HIV-1 infectivity is represented as average fold increase or decrease in luciferase activity relative to control Siren-ve (S-ve) cells (A) or cells transfected with non-specific siRNA (B).

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Virology Journal

ISSN: 1743-422X