Figure 4
From: A dual function TAR Decoy serves as an anti-HIV siRNA delivery vehicle
Inhibition of HIV gene expression by TARmiR constructs. (A) Generation of U6-TARmiR PCR constructs. An PCR expression cassette with the U6 promoter driving the expression of anti-rev TARmiR, anti-TGF-β TARmiR or anti-Rev shRNA is generated as described earlier by us [24]. (B) HEK 293 cells are co-transfected with infectious proviral DNA pNL4-3 and U6anti-Rev TARmiR configuration I, U6 anti-TGF-β TARmiR or U6anti-Rev shRNA. Pronounced inhibition is observed with both, the anti-Rev shRNA and anti-Rev TARmiR. Inhibition is also observed with anti-TGF-β TARmiR. This could be due to the presence of an intact TAT binding bulge which serves in the capacity of a TAR decoy.