Figure 3
From: A dual function TAR Decoy serves as an anti-HIV siRNA delivery vehicle
Gel mobility shift assay: anti-HIV TARmiR configuration I or II was transcribed in vitro, end labeled and was allowed to bind to a peptide corresponding to the arginine rich region of Tat that is responsible for binding TAR [23]. A mobility shift (arrow) clearly demonstrates Tat peptide binding to the TARmiR. As a control the fusion peptide HA-2 of influenza was used to bind the TARmiR configuration I. No shift in mobility is observed with HA2.