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Table 1 Primers used to mutate the capsid protease of Chikungunya virus

From: Functional dissection of the alphavirus capsid protease: sequence requirements for activity

Mutation

Primer

Nucleotide sequence (5'-3')

Wt

Flagchik cap_fwd

aagcttatggactacaaagacgatgacgataagaccatggagttcatccc

EGAEEW (wt)

ChikcapXmaI_WT_rev

atcccgggtccactcttcggctc

Δ46 AA

(deletes aa 216-261)

ChikcapΔ46_rev

atcccgggttctaccgctgtcccc

W261A (EGAEEA)

ChikcapW-A_rev

atcccgggtcgc ctcttcggccc (4)

A258E (EGE EEW)

ChikcapA-E_rev

atcccgggtccactcttcttc cccctcgg (5)

A258 S (EGS EEW)

Chikcap3A-Sr_rev

atcccgggtccactcttcgga cccctcgg (6)

E256A (A GAEEW)

Chikcap_E-A_rev

atcccgggtccactcttcggccccggc (7)

A258T (EGT EEW)

ChikcapA-Tr_rev

atcccgggtccactcttcggt cccctcgg (8)

E260 D (EGAED W)

Chikcap_E-D_rev

atcccgggtccaatc ttcggccc (9)

E260A (EGAEA W)

Chik_cap_E-A_rev

atcccgggtccacgc ttcggccc (10)