Figure 1From: Prediction of conformational changes by single mutation in the hepatitis B virus surface antigen (HBsAg) identified in HBsAg-negative blood donorsAlignment of amino acid sequences of HBV isolates in Indonesian blood donors with frequently-reported variants associated with failure of diagnostic assays. Three amino acid substitutions were identified in 7 HBV isolates in blood donors: Pattern 1, T123A, in one isolate; Pattern 2, M133L, in one isolate; Pattern 3, T143M, in five isolates. HBV DNA isolated from the remaining 18 samples showed wild type (wt) sequences with no amino acid substitution. Consensus of each of the three single mutation patterns and wt were aligned with five known variants frequently associated with problems in diagnostic assays and/or escape to vaccine/HBIg therapy: T123N, M133I, M133T, M133V, and T143L, together with M54923 sequence (genotype B/adw) retrieved from GenBank as a reference.Back to article page