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Table 3 Plasmids involved in hr3 enhancer function in trans

From: Baculovirus immediately early 1, a mediator for homologous regions enhancer function in trans

Plasmid No.

Corresponding site in T3 strain

Intact coding regions contained

CPM

19

114443-119152

odv-e18, odv-ec27, orf-121, orf-122, ie-1

202023

58

115898-119619

orf-121, orf-122, ie-1

554392

186

115898-119152

orf-121, orf-122, ie-1

100268

262

115216-119619

orf-121, orf-122, ie-1

112732

280

114846-120318

odv-ec27, orf-121, orf-122, ie-1, odv-e56, orf-125

381711

289

115216-119619

orf-121, orf-122, ie-1

261497

310

114846-120549

odv-ec27, orf-121, orf-122, ie-1, odv-e56, orf-125

159852

347

115898-119152

orf-121, orf-122, ie-1

717312

  1. All the coding regions were presumed according to the complete genome sequence of the BmNPV T3 strain (GenBank accession no. L33180). Luciferase activity indicated as CPM in 15 sec (minus the background counts of cells). Each reaction contained 10 μg protein extracted from the transfected cells. The CPM values obtained from the transfection of helicase promoter containing reporter plasmid alone were in the range of 18 to 40.