Figure 2From: Baculovirus immediately early 1, a mediator for homologous regions enhancer function in transTransactivation effects of hr3 enhancer sub-regions on different promoters via co-transfection with pGEM-T-ie1 DNA. Reporter plasmids containing different promoter were co-transfected with pGEM-T-ie1 and hr derivates. 0.1 μg of pGEM-T-ie1 plasmid DNA was used in each transfection. Each luciferase reaction contained 10 μg of protein extracted from the transfected cells. CK, no hr added; hr114, plasmid containing a fragment without intact 30-bp incomplete palindrome but half of the palindrome on both sides; hr198 contained one 30-bp incomplete palindrome; hr3 contained an intact 651-bp hr3 fragment with 3 palindromes.Back to article page