Figure 3

HCV-3a Core specific shRNAs inhibit mRNA expression. A) Total cellular RNA extracted from transfected Huh-7 after 24, 48 and 72 hrs post-transfection. Gene expression results from Real Time PCR showed that Csh352 and Csh476 shRNAs against HCV-3a Core decrease RNA expression using gene specific primers in comparison to Mock with GAPDH as internal control. Three independent experiments were performed having triplicate samples. Error bars indicate mean S.D, *p < 0.01. B) Silencing of HCV-3a Core gene by shRNAs using specific antibodies showed reduction at protein expression level determined by western blot analysis after 24, 48 and 72 hrs transfection with Mock (M), HCV-3a Core expression plasmid (C) with and without HCV-3a shRNAs (Csh352 and Csh476) and scramble shRNA (ScshRNA) in Huh-7 cells. Protein levels for GAPDH gene are also shown as internal control.