Figure 2
From: Construction of Metabolically Biotinylated Adenovirus with Deleted Fiber Knob as Targeting Vector
Cell transduction was ablated in the Ad.9RPSTCD-GFP viral vector and was rescued through biotin-avidin-biotin interaction. (A, B) The transduction efficiency of the indicated viral vectors to MA104/APP cells. Cells were collected and GFP-positive cells were counted by flow cytometry 24 h after transduction at different MOI (viral particles/cell). (B) The transduction efficiency of the Ad.9RPSTCD-GFP(avidin) vector to cells increased through the interaction of avidin on the Ad.9RPSTCD-GFP(avidin) vector with the biotin on the cell surface. Indicated viral vectors were added to chemically biotinylated MA104/APP cells (bio+) or unbiotinylated MA104/APP cells (bio-), and the GFP positive cells were counted 24 h later by flow cytometry. The data shown represent means ± SD of triplicate determinations of the GFP positive cells.