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Figure 5 | Virology Journal

Figure 5

From: A Functional Henipavirus Envelope Glycoprotein Pseudotyped Lentivirus Assay System

Figure 5

Cell surface expression of truncation mutants of the henipavirus F glycoprotein. The various F cytoplasmic tail truncation mutants alone or together with their G glycoprotein partner were transfected into HeLa-USU cells. At 24 hr post transfection, cell surface proteins were biotinylated and precipitated with Avidin agarose beads, and the precipitated proteins were processed for Western blot analysis as detailed in the Methods and probed using the anti F1 specific antisera. This experiment was performed twice and representative experiment is shown in the figure.

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