Figure 3
From: A Functional Henipavirus Envelope Glycoprotein Pseudotyped Lentivirus Assay System
Infection specificity of henipavirus F and G bearing pseudovirions. The HeV and NiV envelope glycoprotein pseudotyped virus particles were preincubated with 2 μg of NiV-FC2, Sc-NiV-FC2, soluble, murine ephrin-B2, or soluble human ephrin-B2, mAb m102.4 IgG, recombinant NiV sG, or nothing (control), for 1 hr at 4°C and then receptor positive 293T cells were infected (transduced) with the various treated pseudotyped virus preparations in triplicate wells. After 1 hr incubation, complete media was added and infections were continued for an additional 48 hrs. Cells were then lysed and assayed for luciferase reporter gene activity as described in the Methods. Error bars indicate the standard error of the mean from triplicate wells.