Figure 2

Henipavirus F and G bearing pseudovirus infection assay with GFP-encoding lentivrus particles. 293T cells were co-transfected with the pNL4-3-GFP-E-R⁺ HIV-1 backbone plasmid along with either empty pCAGGs vector, homologous combinations of NiV F/G, HeV F/G, or HeV F with HeV G_SM7. The supernatants were collected 36 hr post-transfection and processed as detailed in the methods. Receptor positive 293T cells were infected with pseudovirions and scored for transduction efficiency by counting the number of GFP positive green cells 48 hr post-infection using Olympus IX81 fluorescent microscope.