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Figure 1 | Virology Journal

Figure 1

From: SARS coronavirus protein 7a interacts with human Ap4A-hydrolase

Figure 1

Co-immunoprecipitation of Ap 4 A-hydrolase and the SARS-CoV 7a protein. HEK 293 cells were transiently transfected with pcDNA3.1/V5-His-Ap4A and/or pXJ-7a-HA. Proteins from cell lysates were immunoprecipitated with monoclonal anti-V5 antibodies (lanes 1 and 2) or unrelated monoclonal anti-β-actin antibody (lane 4). After immunoprecipitation, samples were subjected to 12% SDS-PAGE, transferred to nitrocellulose membrane and probed with monoclonal anti-HA antibodies. Lane 3 represents Western blotting of cell lysates without immunoprecipitation as a control.

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