Table 1 Primers for amplification of DPOL, pTP and hexon gene sequences

Degenerate primers

DPOL-cons

DPOL

4431-s^$

GTnTwyGAyAThTGyGGhATGTAyGC

4428-as

GAGGCTGTCCGTrTC(n/i)CCGTA^#

956 bp

45°C

4428-s

CGGACGCCTCTGyTGGAC(n/i)AA

4429-as

GGCCAGCACrAA(n/i)GArGC

650 bp

45°C

Hex-5'-cons

Hexon

4515-s

GTGGATGG(n/i)GA(r/i)GG(n/i)TACA

4515-as

CGCACAACGTC(r/i)AA(n/i)AC(y/i)TC

536 bp

45°C

4516-s

TGTAACATGAC(y/i)AA(r(i)GA(y/i)TGG

4516-as

CAGGGCCCCCAT(n/i)GACA

381 bp

45°C

Hex3'-cons

Hexon

4517-s

CGCAATGGTC(n/i)TACATGCAC

4517-as

CAGTGCCCGA(r/i)TA(k/i)GG(n/i)TT

340 bp

45°C

4518-s

GCAGGACGC(y/i)TCGGAGTA

4518-as

CACCC(k/i)GTT(r/i)TC(n/i)CC

230 bp

45°C

pTP-cons

pTP

4521-s

TGGCGACGT(n/i)GT(n/i)TACAG

4521-as

CGGACT(y/i)(k/i)GA(r/i)CCTGAAA

260 bp

45°C

4522-s

TACAGCCG(n/i)GTSTGGAAC

4522-as

CTGAAAGAGAGTTC(n/i)ACAGAATCA

230 bp

45°C

Specific primers for long-distance PCR

pTP-DPOL-LD

pTP, DPOL

4659-s

TCGCATCTCCAACGACCT

4659-as

GCATCCATGGTGAAGATTCC

2350 bp

60°C

Hex-LD

Hexon

4618-s

AGTTCGCTACACACTGGCTG

4618-as

ATTGCGGTGATGATTGAATG

1354 bp

59°C

pTP-Hex-LD

pTP, Hexon

4662-s

CTCGGTATCGTTGACGGC

4662-as

GATCAACGGGCACAAAGC

10044 bp

60°C

Primers specific for AdV species B

Hex-loop2

Hexon

5442s

GAACAAGATACTTTAGCATGTGGAA

5442as

GATTGAATGGATTAACATTGTCC

468 bp

55°C

Hexon

5443s

TAGAAAATCACGGGGTGGAAGA

5443as

GGCATCCAAAGACCATCTG

380 bp

55°C